Factors Affecting the Cellulolytic Activity of Rumen Contents
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منابع مشابه
Factors affecting the cellulolytic activity of rumen contents.
The cellulolytic activity of rumen contents was assayed by measuring losses in weight and tensile strength of cotton yarn incubated in rumen contents in the presence of dietary additives (barley, tallow) and at different pH values. The addition of barley depressed cellulolysis and the titer of filter paper-degrading bacteria only if the pH was allowed to fall. Lowering the pH from 7.0 to 6.0 by...
متن کاملFibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents.
Four sheep were fed an alfalfa hay diet. Rumen content samples were collected three hours after feeding in order to total microorganism population (TP), solid attached population (SAP) and solid attached firmly population (SAFP). Fibrolytic specific activities (xylanase, CMCase and beta-glycosidases) were estimated by the amount of reducing sugars or p-nitrophenol released from the appropriate ...
متن کاملFactors affecting the activity of cellulases isolated from the rumen digesta of sheep.
Sodium phosphate buffer was used to extract cellulases from the plant solids fraction of rumen contents. The mixed cellulase preparation had maximal activity at pH 6.9 and 49 degrees C. The V(max) and the apparent K(m) for wheaten hay cellulose were 19.8 glucose units/min and 6.35 mg/ml, respectively, and for microcrystalline cellulose (Sigmacell) at the same enzyme concentration, they were 33 ...
متن کاملSubstrate specificity of rumen cellulolytic enzymes.
Atkinson, B. & Green, A. L. (1957). Tran. Faraday Soc. 53, 1334. Balls, A. K. & Jansen, E. F. (1952). Advane. Enzymol. 13, 321. Childs, A. F., Davies, D. R., Green, A. L. & Rutland, J. P. (1955). Brit. J. Pharmacol. 10, 462. Cohen, J. A., Oosterbaan, R. A., Warringa, M. G. P. J. & Jansz, H. S. (1955). Disc. Faraday Soc. 20, 114. Cunningham, L. W. (1954). J. biol. Chem. 207, 443. Cunningham, L. ...
متن کاملThe Cellulolytic Activity of Pure Strains of Bacteria from the Rumen of Cattle.
The in vitro breakdown of degraded and undegraded varieties of cellulose was examined by using pure strains of bacteria isolated from the rumen of cattle. One strain of Bacteroides succinogenes, two strains of Ruminococcus albus and two strains of Ruminococcus jlavefaciens were allowed to ferment ground cellulose powder (prepared from filter paper), cellulose powder (Whatman) and undegraded cot...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1977
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.33.3.497-502.1977